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The stability of mixed probes kept at 4°C and used for melting point determination was evaluated using 36 independent capillaries and running six new capillaries after 0, 4, 24, 48, 120 and 192 hours.
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The results of the fivefold determination were evaluated using MS Excel Solver.
The method precision of the proposed AuNPs- luminol-ferricyanide SIA-CL method for determination of NLX was evaluated using intra-day and inter-day terms.
The lower limit of detection of the proposed SIA-CL method for determination of NL was evaluated using signal-to-noise ratio.
Three minutes after the tension-frequency determination, fatigue resistance was evaluated using a low-frequency fatigue protocol of 50 hz trains of 700-ms delivered every 2 s for 5 min [36].
The potential for the rapid, high throughput determination of urinary creatinine was evaluated using replicate aliquots of diluted urine, mixed with acetic anhydride prior to injection at one minute intervals and analysis by R-TD-EESI-IM-MS/MS.
Finally, the antibacterial activity was evaluated using MIC determination on four Gram-positive cocci (Bacillus subtilis, Clostridium difficile, Enterococcus faecalis, Staphylococcus aureus), three Gram-negative bacilli (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae), and the yeast Candida albicans.
Calibration models were developed with partial least squares (PLS) regression and model performance was evaluated using coefficient of determination (R2) and root mean square error (RMSE).
Determination of total glycosaponin contents in each extract was evaluated using the gravimetric [ 2] method with slight modification.
Goodness of fit was evaluated using the coefficient of determination (r) between the observed and estimated nest numbers to facilitate comparisons with a previously published method [ 19].
Subsequently, the area of GFP fluorescence throughout the stack was evaluated using the ImageJ software for area determination.
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