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To compare the difference between central tendency of results, the coefficient of determination was calculated using statistical software and an ANOVA test was performed.
Sample size determination was calculated using Power and Sample Size Program (PS) package (version 3.0.43).
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Coefficients of determination were calculated using Excel (Microsoft Corp., Redmond, Washington).
The mRNA half-life determinations were calculated using the one-phase exponential decay method (22) using GraphPad Prism software (GraphPad Software, San Diego, CA).
The photon intensity and spectrum for the source at the RAKR determination point was calculated using MCNP code version 5 [ 20].
Three experiments were conducted for each determination and significance was calculated using Student's t-test.
Three experiments were conducted for each determination, and significance was calculated using the Student's t-test.
Determination of symmetry was calculated using the symmetry index (SI) given by (1) S I = X A X N A, where XA is the gait variable recorded for the limb affected by pain and XNA is the corresponding variable for the nonaffected limb.
Determination of relative expression levels was calculated using GeNorm normalization factor including the three most stable out of six reference genes [ 25].
The mean analytical recovery was calculated using five determinations and was found to be 98.74 ± 0.7% to 100.22 ± 1.24% indicating the accuracy of the proposed assay.
The gene expression level was normalized using GAPDH as an internal reference gene, and the average relative change was calculated using 3 5 determinations by relative quantification, applying the delta-delta cycle threshold method.
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