Sentence examples for determination virus from inspiring English sources

For nucleic acid type determination, virus replication was tested in DEF cultures with or without the presence of BrdU in the medium.

According to virus titer determination virus productions ranged between 10 and 10 TU/ml and viral infection of T47D breast cancer cells was performed using previously described protocols [ 16– 16].

For an exact determination of virus RNA concentrations in the 12 samples, 12 individual probe-based real-time RT-PCRs were designed upon determined virus sequences (Table).

Determination of virus sequences also now opens the way to more detailed studies of virus gene function.

We therefore present a bundle of real-time PCR assays which enable a fast and precise determination of virus type and subtype in respiratory specimens, including the new influenza A(H1N1)v virus.

Until 2008 2009, laboratory confirmation essentially relied on enzyme immunoassays for determination of virus type, and isolation in cell culture followed by hemagglutination inhibition assays using specific polyclonal sera for determination of virus sub-type and antigenic characterization.

At this time, plates were frozen for subsequent determination of virus titers.

Observation of plaque morphology and the determination of virus titer were performed by plaque assay as described earlier [37].

For determination of virus titer, serial dilutions of each sample were made by mixing 45 µl of lysate in 405 µl of M199 media.

For the determination of virus titers on the indicated days post-infection, the ovaries were removed and homogenized in medium using a Tissue Lyser (Qiagen), and the spleens were made into a single cell suspension between two frosted slides and resuspended in 10 ml complete RPMI-1640 medium.

For determination of virus loads in macaque blood and tissue samples, Vero E6 cells were seeded in 48-well plates the day before titration.