Supernatant (25 µl) was used for ATP determination following the procedure provided in the Sigma FL-AA Bioluminescent assay kit.
Ascorbate determination followed the procedure described by Law et al. (1983).
The colonisation of the wood specimens was not confirmed by mass loss determination because the procedure required crushing and destruction of the samples.
The assay for protein concentration determination followed the procedure of Tsai and Lin Tsai and Linn, 2007; Tsai and Lin, 2012) with the BioRad protein assay kit (cat. no. 500-0002, BioRad, USA).
For all automated steps, including plate setup and inoculation as well as agar plating for MBC determination, the manual procedure required 68 min of pipetting (spread over 2 days), while the automated procedure took only 22 min (also over 2 days) and did not require the constant attention of laboratory personnel.
Though the government has tried to outlaw the prenatal determination of sex, the procedure is readily available at countless small-town hospitals and the growing number of for-profit "maternity clinics".
This enables the technique to be used more frequently in clinical practice as it reduces the cost of GFR determination, and allows the procedure to be undertaken easily in an outpatient setting.
In these experiments, five iterations are used in the initialization estimation and order determination according to the procedure in Figure 4, consuming CUP time of coarse motion estimation corresponding to different SNRs is 1.13, 1.16, 1.86, and 2.17 s, respectively.
The ICC of the threshold determination procedure of the first series of experiments, which was calculated over the three trials that were used for each threshold, was.91 (confidence interval.80 .96) for the needle electrodes and 1.00 (confidence interval.99 1.00) for the disc electrodes.
