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And although he would not acknowledge the extent of Leinart's injury — he said the team doctors did not think the X-rays were clear enough to make a determination — the expression on his face showed that the Texans might have finally sustained the injury that would throw them off kilter.
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Since TTHA1913 could be expressed and purified from E. coli for structure determination, whereas the expression of the B. pseudomallei HicA protein in E. coli was toxic [ 21, 27], it seems likely that TTHA1913 is a non-toxic HicA homologue.
The determination of the expression level of the key genes is a good opportunity to improve a sound understanding of the high temperature bio-assisted heap leaching of chalcopyrite and to optimize the technology.
In this work, a microfluidic paper-based fluorescent sensor device was constructed for quantitative detection of microRNA (miRNA) and determination of the expression of folate receptor (FR) on the cell surface.
The simultaneous determination of the expression status of multiple, clinically relevant tumour markers could lead to a better detection of the primary tumour and its metastases, providing optimal tumour delineation during surgery under image guidance.
An important implication of this result is that it enables the simultaneous determination of the expression status of two tumour markers, both in a non-invasive manner in vivo, as well as ex vivo.
After determination of the expression of the transduced genes using RT-PCR and Western blot (Figure 1A), cells were differentiated as previously described [16] (Figure 2).
Specifically, this method could allow the accurate determination of the expression levels of multiple transcripts that could lead to the false positive of an expression differential by hybridization due to the high identity at nucleotide level.
MiScript primer assay (Qiagen) was used for semiquantitative determination of the expression of human miR-155 (MS00003605).
Although the determination of the expression levels was beneficial, RAD51 expression was not affected by either PTEN mutation or loss of PTEN expression.
This allows the determination of the expression of ORF1, as well as the ORF1a and ORF1b variants (sharing the N-terminal epitope).
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