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The extraction was filtered through a Whatman No. 1 paper (Whatman Inc., Shanghai, China) and the supernatant was used for phenolic contents determination, the content of total phenolic was expressed as gallic acid equivalent on a FW basis.
The standard curve of Fe with R2 = 0.9999 is used for determination the content of Fe2+ and total iron with 2.5 mL 0.01% (w/v) SPION digested by HCl, pH = 4.30 HOAc-NaAc buffer 10 mL, 0.01% (w/v) 1,10-phenanthroline 5 mL and 10% (w/v) ascorbic acid 1 mL for total iron determine independently.
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A simple and reliable high performance liquid chromatographic method was developed for determination the contents of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D glucopyranoside (THSG), emodin and physcion, which were considered to be potent active ingredients.
The type of gasifying agent is a key factor in the determination of the content of the hydrogen product.
The FRB extracts were used for the determination of the content of anthocyanins and phenolic acids by reversed-phase HPLC as described previously (Pengkumsri et al. 2015).
A method is developed for the determination of the content of the active component in a pharmaceutical preparation by means of PLS calibration.
Biosensors for the determination of the content of plant secondary metabolites in nutraceutical preparations have also been reported for glycoalkaloid compounds.
The resulting chromatogram identified each monoamine position and concentration from the sample as compared to that of the standard, and finally, the determination of the content of each monoamine as μg per gram brain tissue [ 29].
Our portal was designed with a strong focus on the end-user, in which patients were invited to be actively involved in the determination of the content and the design of the portal.
In the European standard EN 16143 [ 27], a two-step sample preparation procedure for the determination of the content of benzo[ a]pyrene and seven other PAHs is used.
Therefore, the determination of the content of 33EPs in feces could be used to monitor gluten detoxification of celiac patients treated with novel enzymatic therapies (3, 21, 23), proving the utility of these methods in experimental therapies for CD.
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