Sentence examples for determination substrate from inspiring English sources

For pH optimum determination, substrate was dissolved in 50 mM Tris-acetate buffer containing 150 mM NaCl at the indicated pH values.

According to [ 35] and [ 36], six functional domains in the F3'5'H enzyme are important for the determination of substrate specificity and 3' vs. 3'5'-OH activity (substrate recognition sites, SRS; candidate region, CR1).

Cyclodextrin (α, β, and γ) substrates and pullulan were not cleaved by the enzyme (Table  2).> -wrap-foot> For the determination of substrate specificity, recombinant enzyme was incubated for 5 min in Tris HCl buffer (50 mM, pH 7.0) at 80 °C with different substrates (0.5%% w/v) The enzymatic activity of α-amylase was tested in the presence of NaCl (0 25 % w/v).

A PHOTOMETRIC method for chicken liver xanthine dehydrogenase based on the colorimetric determination of substrate disappearance1 has been developed.

Our work aimed at finding out the region responsible for determination of substrate specificities of these two urdamycin GTs.

These results clearly demonstrate the importance of remote residues, not readily predicted by rational design, for the determination of substrate specificity.

An optimisation procedure based on genetic algorithm approach is developed for the determination of substrate feed profiles for the optimal operation of fed-batch bioreactors.

Accordingly, secondary structure has relatively more impact on the determination of substrate and product.

Microsomal fractions from tobacco leaves expressing GALT2-GALT6 constructs were used for determination of substrate specificity as described by [ 15, 16].

Especially problematic is the determination of substrate specificity of the enzymes active on the structurally most complex amylopectin and glycogen molecules.

We have also determined that the structural determinations of substrate specificity are both indirect and subtle: a Pro/Ala substitution appears to be the major determination of specificity.