Sentence examples for determination reactions from inspiring English sources

For amplification efficiency determination, reactions were carried out in triplicates.

The SNP determination reactions were conducted for CYP4D4v2, CYP4G2, and CYP6A38 using allele specific primers, SNP450HF5F, SNP450HF20F, and SNP450HF17F, respectively (Table 1, Fig. 7), designed according to the sequences immediately upstream of the nucleotide polymorphism to distinguish the single nucleotide polymorphism for the P450 allele in each house fly strain or line.

The SNP determination reactions were conducted for each of the genes using a specific primer (Fig. 7) designed according to the sequences immediately upstream of the nucleotide polymorphism in order to distinguish the single nucleotide polymorphism for the P450 allele in each house fly strain or line.

Such data can then be exploited in model building and checking pipelines such as the SuBliMinaL Toolbox [22], which include automated methods for metabolite charge state determination, reaction balancing and model merging.

For IC50 determinations, reaction mixtures with various concentrations of WDR5-0103 (1 μM–1 mM) were incubated for 1 h at room temperature and then quenched by adding 20 μl of 7.5 M guanidinium chloride, followed by the addition of 180 μl of buffer (20 mM Tris/HCl, pH 8.0).

Open image in new window Fig. 5 Graphical analysis for the determination of reaction order and reaction rate constants: (a) zero-order reaction, (b) first-order reaction, and (c) second-order reaction.

Figure 5 represents the graphical analysis for determination of reaction order and reaction rate of the process using above equations.

For serogroup determination, amplification reactions (50 μL) contained 15 μL of DNA, 60 mmol/L Tris-HCl (pH 8.8), 17 mmol/L (NH4 2SO4, 5 mmol/L MgCl2, 0.5 mmol/L of each dNTP, 0.3 μmol/L corresponding oligonucleotides, and 1 U of Taq polymerase.

Of special concern is the effect of the above processes on experiments in which low-yield reaction products are of interest, such as determination of reaction kinetics and isotopic fractionations during pyrite oxidation reactions.

The identification of the dominating skeleton subnetwork such as the MPU and key feedbacks in a regulatory pathway also simplifies the determination of reaction rates of in vivo biochemical reaction since the distracting unimportant reaction components have been removed from the skeleton structure [ 45, 46].

This lack of information propagates as uncertainty in the estimation of fluxes and basic reaction properties such as the determination of reaction directionalities.