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SGs hydrolysis was also analyzed by a SPE-GC-FID based determination previously described [ 33].
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The TP values obtained for these intact leaf measurements here, are very similar to various determinations previously conducted on some of the lines using isolated chloroplast preparations or detached whole leaves [ 29, 36, 37].
These inoculation conditions equate to an effective MOI of 1.0 0.5 as determined previously via TCID50 determination in HeLa cells.
The iterative process for large-scale RLS determination was previously described [15].
Thus, the DNA data is in concordance with the anthropological sex determination performed previously.
Injured cortical areas, i.e. unstained tissue as a reflection of cell loss (Figure 2B), were measured using the computer image analysis system (Scion, NIH software, Bethesda, USA) and the distance between respective coronal sections were used to calculate a linear integration for the lesion volume determination as previously described [42].
The sample size determination is previously described.
The method for manual TAC plasma determination was previously described by Lusignoli et al [ 18].
Tissues were processed for adipocyte size determination as previously described [ 18].
Expression of CD137 was determination as previously reported for T cells [ 5].
Lentiviral production and titre determination were previously described (Liu et al, 2006).
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