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Is the same gene used in other dipteran sex determination pathways as a male determining locus?
Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element.
By contrast, stem cells maintain this flexibility, enabling them to embark on different determination pathways.
However, it is now known that only parts of the Drosophila sex determination pathways are conserved in other insects and the Tephritidae family affords an example.
In fish, it has been implicated in male sex determination pathways in the Nile tilapia Oreochromis niloticus [26] and rainbow trout Oncorhynchus mykiss [27], and shown to contribute to both male and female gonad development in zebrafish [28].
Additionally, it would be of interest to follow expression of figla and of the other sex determination candidates quantitatively, at different stages of gonad development, as timing and expression levels of genes in sex determination cascades have been shown to be critical to promote the switch between male and female determination pathways [62] [65], [73].
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These results are consistent with the hypothesis that sex-specific selection acts on alleles linked to the male-determining locus driving evolutionary turnover in the sex determination pathway.
We find that the PcG/Trx-G proteins genetically interact with mutations in the sex determination pathway and influence the ability of females to determine their sex.
Recently, the nematode sex determination pathway was also implicated in vulval fate determination [55].
However, the Drosophila sex determination pathway is only partially conserved and the family Tephritidae affords an interesting example.
fruitless is a key component of the sex determination pathway encoding sex-specific and non-sex-specific proteins that are members of the BTB-Zn finger transcription factor family [42], [71].
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