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For the genomic DNA extractions (gDNA), hemocytes were recovered individually as described above from three oysters used for gene quantification and from one oyster used for gene structure determination originating from the Mediterranean Sea (Thau lagoon).
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If the regulation of sex determination originates upstream, then the B class genes clearly are key integrating points in the regulatory cascade.
While we have identified SpPI and SpAP3 as a key factors in both floral organ identity and sexual dimorphism in spinach, it is likely that regulation of sex determination originates upstream of the floral organ identity genes.
Lifespan determination was performed with cultures originating mono-nucleate ascospores (wild-type: n= 66, Δ PaCatB: n=20, PaCatB_OEx1: n=29, PaCatB_OEx2: n=11, PaCatB_OEx3: n=14).
All determinations were made from kits originating from the same lot.
As each crystal can usually yield only a single diffraction pattern, structure determination is only possible by merging data originating from hundreds of individual crystals.
The developed method was successfully applied to the simultaneous multielemental determination of hydride forming elements in spring water samples originating from two different regions in Hungary.
This technique in particular permits the characterization of the organic components by the determination of the content of amino acids and fatty acids originating from proteins and fats respectively [8 10].
Practical diagnostic applications of such assays demonstrated in this study include: (1) rapid genotype determination of isolates; and (2) identification of mixed-genotype isolates originating from pooled samples in areas where genotype distribution is known to overlap.
Present study aimed at determining prognostic and predictive value of immunohistochemical determination of ER, pS2, MT, and CD24 expression in sections originating from 104 patients with breast cancer.
originating from the actively growing part of the fungal colonies were used to inoculate either solidified OMW media (for the purposes of mycelium growth rate determinations) or static liquid cultures (for OMW degradation experiments with selected strains).
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