Exact(5)
The minimum bactericidal concentration was determined by subculturing aliquots from MIC determination onto substance-free agar plates.
The minimum bactericidal concentration (MBC) and minimum fungicidal concentration (MFC) of the synthesized benzimidazole derivatives was determined by subculturing 100 µl of culture from each tube that remained clear in MIC determination onto sterilized petri-plates containing fresh agar medium.
The subculturing of 100 µl of culture from each tube that showed no growth in MIC determination onto sterilized petri-plates containing fresh agar medium gave the minimum bactericidal concentration (MBC) and fungicidal concentration (MFC) of the synthesized compounds.
The MBC values were obtained by subculturing the contents of each negative well and from the positive control (broth with inoculum, without compound) of MIC determination, onto substance-free Mueller-Hinton agar (MHA) Petri dishes.
The MBCs (i.e., the lowest concentrations of antibacterial agents required to kill a particular bacterium) were determined by subculturing aliquots (20 μL) from wells with no visible bacterial growth and from control wells of MIC determination onto substance-free Mueller-Hinton agar (MHA) plates.
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