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High Content Analysis algorithms detected the number of HepG2 cells with a consistency of 88%, as checked by visual cell limit determination on sample images representing different cell densities and toxic levels, by detecting the number of Hoechst stained nuclei per spot and subsequently using the red labeled actin signal to segment cell contours.
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Determination of sample size was based on a proportionate to size sampling formulae (Groebner and Shannon 2005).
Determination of sample size was based on our previous study in which we detected a difference in insulin sensitivity between 13 MA and 13 NHW study participants, and the study by Houmard et al in which insulin sensitivity was improved by 7 days of cycling exercise in nine sedentary men [3], [24].
The determination of sample size was based on practical requirements rather than any formal statistical sample size calculation.
We provide guides on searching the stopping and rejecting regions and determination of sample size.
A simple molecular approach based on allele-specific PCR (AS-PCR) in combination with sequence analysis for haplotype determination on individual samples was reported in previous studies.
Based on determinations of sampling error and coefficient of variation, twofold differences in gene expression in serial biopsies can be detected by assaying approximately six synovial tissue biopsies from 8to1010 patients.
For c-fos, egfr and HIF-1alpha determination on in vitro samples and FNAs from mice tumours, quantitative PCR was performed on an MX3000p thermal cycler (Stratagene, Lajolla, CA, USA) using SYBR green dye method to track the progress of the reactions with ROX dye added as reference.
Radionuclide determinations on soil samples showed that the highest specific activities (Bq kg−1) were present on a small (0.44 ha) area with a relatively high erosion rate.
New age determinations on nine samples collected from Bîr Sahara East and Bîr Tirfawi are presented for the five paleolake phases originally described by the excavation team.
The accuracy of the method has been validated through the analysis of seawater reference standards and comparison with ICP-MS determinations on seawater samples collected in the upper 1300 m of the subtropical south Indian Ocean.
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