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For determination of viability of LAB, a series of dilution were prepared with peptone water (0.1% peptone).
For determination of viability, the cells were stained with 5 µg/ml of propidium iodide (PI; Invitrogen) and analyzed by flow cytometry.
For determination of viability, 1G5 cells were seeded in 12 well plates at 1×106 cells/well, treated with increasing concentrations of PA analogs, from 100 to 10,000 nM (0.1 to 10 µM), as indicated.
For that purpose, both assays were used for the determination of viability and EC50 values.
For flow cytometry-based determination of viability, cells were harvested, collected by centrifugation, washed once and resuspended in staining buffer (1 × PBS, 10% FBS).
Medical determination of viability is based in empirical science but beliefs about the significance of viability are influenced by morality, culture and politics.
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The determination of cell viability was accomplished on cell viability analyzer (Vi-CELL XR, Beckman Coulter, Fullerton, CA, USA) using elimination test with trypanose blue.
One sample of the particular type was used for the determination of the viability of the cells [9].
The determination of cell viability depends on these physical and biochemical properties of cells.
Determination of cell viability was performed by using MTT assay (Sigma, St . Louis MO), which was used to calculate the growth inhibition induced by increasing concentrations of drug.
However, the natural strong fluorescence of QDs could interfere with signals during the determination of cell viability and create inaccuracies in the obtained data.
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