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The method for each disinfectant and test bacterium was repeated at least four times, when bacterial cultures were exposed to four different samples of diluted disinfectant, before the determination of D-values by the survivor curves.
Using a custom Fiji/ImageJ plug-in, we demonstrate an improved determination of GP values by modeling the spectral data at each image pixel using a Gaussian or Gamma Variate distribution.
Briefly, the results of each assay were validated by determination of EC50 values produced by the standard antimalarial drugs against CQ resistant P. falciparum.
The determination of grayscale value was processed by Image J.
While largely accepted, the determination of this value may be affected by artifacts from histology preparation such as tissue shrinkage, and selection bias (this cut-off was derived from plaques that had already ruptured).
The advantages were presented of iterative method for determination of background values proposed by the authors.
On Locke's view, though the value-mode is determined immediately by the affective character of the value, this is not the final determination of the value.
A small sensitivity (e.g. < 0.1) is indicative of loose determination of the parameter value by the experimental data.
Descriptive analysis was performed by determination of mean values, standard deviations, minimum and maximum values.
3. Determination of realized value.
Fig. 4 Determination of τ value.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com