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Total sulfurs of the samples were determined according to ISO 351 1996 (Determination of total sulfur-High temperature combustion method).
Determination of total bilirubin was determined by the method described by [ 75].
Total moisture was determined following ISO 589 2003 (Hard coal- determination of total moisture).
The determination of total phenolic contents in ceSb was determined by the Folin Ciocalteu method with slightly modifications.
Determination of total protein was performed using biuret method.
The determination of total phenol and flavonoid substance were contemplated.
Standard Test Method for Determination of Total Knee Replacement).
The Kjeldahl digestion procedure was followed for the determination of total nitrogen (Bremner and Mulvancy 1982).
Dried shoot material was milled for later determination of total N and P concentration.
Cold vapour AAS was used in the determination of total dissolved mercury.
GBC AAS932 equipped with the HG3000 hydride generator was used for the determination of total As.
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