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The determination of tolerance indices followed the methods described by Baker & Walker [ 45], and were calculated based on relative root growth (lengths) generated in parallel treatment and control units.
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The sucrose tolerance test was carried out using the same procedure as for the determination of starch tolerance.
The oral glucose tolerance test was also carried out using the same procedure as for the determination of starch tolerance, but glucose at a dose of 2 g/kg body weight was used instead of starch.
Two lines, N4C-1 and N4C-2, were subsequently subjected to phenotypic comparison, germination test, ABA assay, analysis of post-germination growth arrest, and determination of drought tolerance.
After incubation of all the sets, 100 µl of inoculum from each set was inoculated on YPD agar medium for the determination of percent tolerance of yeast isolates (Syal and Vohra 2013).
Regardless of the GCT result, all study participants then undergo a 3-h 100-g OGTT for determination of glucose tolerance status in pregnancy.
Regardless of the GCT result, all study participants undergo a 3-h, 100-g OGTT for determination of glucose tolerance status in pregnancy.
In this study, regardless of the GCT result, all participants underwent a 3-h 100-g OGTT for determination of glucose tolerance status in pregnancy.
In October, before start of the warming treatment, sampling of shoots for determination of freezing tolerance was carried out once and twice in 'Titania' and 'Narve Viking', respectively, because slightly less plants of 'Narve Viking' than 'Titania' were available.
At this stage, the determination of the tolerance on experimental parameters can be evaluated: it is actually the size of the last domain [ 38] (i.e. the minimum and maximum on the x-axis of each histogram).
Our findings may have important implications for determination of glucose tolerance based on results from the postpartum OGTTs, including the diagnosis of diabetes as well as glucose intolerance (prediabetes).
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