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We also thank Dr Thomas Jaenson at Uppsala University for species determination of the ticks.
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The levels of sequence similarities that we found did not enable species determination of the tick in this study.
Using commercially available software designed for the identification of bacteria, a reference database of spectra was constructed that allowed the species determination of ticks using larvae, nymphs, or adult individuals as starting material.
In contrast, we identified 2 cases of human infection with R. raoultii in China by molecular detection and sequence determination of the pathogen in blood samples and from a tick detached from 1 of the patients.
"I am also afraid of the ticks".
Sex of the ticks was ignored.
For molecular identification of the ticks, the tick 16S rRNA gene was amplified and sequenced [ 24].
This associates the tick value of the tick session with the UTC time interval to.
Two larval ticks, 1 nymphal tick, and 2 adult ticks were processed individually for rRNA extraction and PCR amplification of the tick mitochondrial 16S rRNA gene.
We tested amplifiable tick DNA by PCR of the tick mitochondrial 16S rRNA gene (2 ).
Do not dispose of the tick.
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