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However, varying weather conditions throughout the year and the exact determination of the release point on the plot plan and the release elevation are problematic; these issues cause the results to be non-exact and non-integrated.
Hydrolysis of proteins was measured by the determination of the release of a-amino groups directly in milk by the trinitrobenzenesulfonic acid (TNBS) method (Polychroniadou 1988), in which free amino groups react with the TNBS reagent (Sigma-Aldrich, Bornem, Belgium) at pH 9.2 in the absence of light.
Determination of the release characteristics of AC from the herb-loaded CA fiber mats was carried out by the total immersion and the transdermal diffusion through a pigskin method in acetate or phosphate buffer solution that contained methanol (hereafter, A/B/M or P/B/M medium) at either 32 or 37 °C, respectively.
The determination of the release of P. pentosaceus SB83 from vaginal tablets was done using simulated vaginal fluid (SVF).
The rapid determination of the release of structural sugars from biomass feedstocks is an important enabling technology for the development of cellulosic biofuels.
Conversely, their action was virtually irreversible, and therefore, probably indicative of tight, yet noncovalent binding of the inhibitors.[ 16] The determination of the release rates (off-rate) of the inhibitors from the enzyme was, however, hampered by the rapid loss of the enzymatic activity in a diluted solution.
Similar(54)
β-Glucosidase (BGA), alkaline phosphatase (APA) and arylsulphatase (ASA) enzyme activities were assayed by incubation of samples with p-nitrophenyl glucoside (0.025 M), p-nitrophenyl phosphate (0.115 M) and p-nitrophenyl sulfate (25 mM) as described by Eivazi and Tabatabai [34, 35] and Tabatabai and Bremner [36] followed by the spectrophotometric determination of the released p-nitrophenol.
β-glucosidase activity was assessed by determination of the released p-nitrophenol, after the incubation of samples (1 g fresh weight) with p-nitrophenyl glucoside (0.025 M) for 1 hour at 37°C, in a Bio-Rad Microplate Reader at 400 nm [44].
Determination of the released reducing sugars is performed after each step of the procedure.
It is worth noting that the MALDI-TOF oligosaccharide profiles do not allow the unequivocal structural determination of the released oligosaccharides, essentially because of the isobaric nature of Xyl p and Ara f.
Our results allow a qualitative determination of the potential release of ENMs into technical or environmental compartments, with the highest potential release expected during recycling.
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