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The combination of PTR-MS, platinum catalyst and sensitive CO2 detector allowed for real-time VOC sampling with a quantitative determination of the compounds produced.
It has been extensively used in the determination of the compounds of pharmaceutical importance applied to their kinetic studies (Rahman & Azmi 2001a; Campins-Falco et al. 1996; Arayne et al. 2008).
A secondary BMV assay guided fractionation of supernatant extract from surviving colonies led to fractions with high antiviral activity, which were then further fractionated for structural elucidation and activity determination of the compounds.
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The synthesis and X-ray determination of the compound 2 was reported by Solak et. al. [7] but the spectral studies of the compound 2 were not.
In addition, the determination of the compound's concentration and the low efficiency of removal methods make it difficult to establish a complete database of HMF concentrations in different monofloral and multifloral honeys.
HPLC conditions, particularly the mobile phase and its elution program, are important for determination of the compound in the biological matrix.
This instrumental configuration minimizes sample manipulation and allows the determination of these compounds without the need for a derivatization step so that the methodology is largely shortened and simplified.
A current research topic is the determination of these compounds and the effectiveness of some solvents [ 4].
The same system can also be applied for the quantitative determination of these compounds, but only for the more soluble ones.
Octadecyl column is the most commonly used in the determination of these compounds [ 3– 16].
The method was applied to the determination of these compounds in canned soft drinks and canned foods.
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