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For determination of sphere size, we gauged sphere diameter optically with an object micrometer.
Proliferation was assessed with a combination of CellTiter-Blue Assay (Promega), which measures mitochondrial reductase activity, and microscopic determination of sphere diameter as described previously (by Moors et al. 2009).
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However, very good agreement can be found for the spectral positions of the Mie oscillations which is important for a correct size determination of the sphere.
To illustrate the technique for the determination of numbers of spheres, let us suppose that we have a homogenous population of spherical bodies in the cytoplasm.
The determination of suitable variable hard sphere (VHS) molecular model parameters for DSMC simulations using measured growth rate distribution is demonstrated with aluminum vapor as an example.
Please note that the following objects were not included in our analysis: (a) sphere S0 was excluded due to its very small size, (b) bottle C1 was not analyzed because we discovered that a large error was made in determination of its activity, and (c) sphere S1 was not analyzed because it was not visible in images reconstructed from scans with hot water.
DLS allows determination of hydrodynamic diameter of colloidal particles and conjugates, that is the diameter of sphere with the same Brownian motion as the analyzed particle.
The solvent probe radius specifies the size of water spheres for the determination of the solvent space and is typically set to 1.4 Å for water.
The analysis of light scattered by homogenous polystyrene spheres allows the determination of their diameters using Mie theory.
Quantification of sphere growth and passaging.
This allows for a ready determination of both primary collection (particle deposition on the collecting sphere) and secondary collection (particle deposition on previously deposited particles).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com