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Because the latest systems allow the determination of sequences up to 1100 nt in length (e.g. the PacBio sequencer and 454 GS FLX+ system; http://454.com/products/gs-flx-system/index.asp, accessed on 11 November 2013), the excluded sequences might be useful as primer candidates in the near feature.
In Oryza, FISH mapping of pachytene chromosomes resulted in the construction of a high-resolution map that allowed the determination of sequences at a resolution of ∼40 kb.
Future determination of sequences from complementary DNA (cDNA) and/or proteins will help to substantiate the putative translation start positions as well as RNA editing sites.
Much of the interest in characterizing heparin structure is focused on the determination of sequences that interact with proteins and peptides.
Sequencing reactions were carried out using the Dye Terminator Sequencing kit for automatic determination of sequences with ABI PRISM 377 (Applied Biosystems, Foster City, CA, USA).
The preparation of the template DNA and the determination of sequences of each gene followed the procedures described previously (27 – 27 ).
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Primers, determination of sequence alleles, and designation of sequence types (ST) are described on the MLST web site (http://neisseria.org/nm/typing/mlst/).org/nm/typing/mlst/
A preliminary alignment of the proteins was used to identify sequence gaps that were removed prior to the determination of sequence divergence.
It implemented the Needleman-Wunsch algorithm [3] for the determination of sequence homologies in order to be able to perform rapid homology measurements on large numbers of sequence pairs.
Primers, determination of sequence alleles and designation of sequence types were those described on the MLST website http://neisseria.org/nm/typing/mlstdb/.org/nm/typing/mlstdb/
This amino acid contributes to the determination of sequence specificity by the homeodomain, suggesting that these proteins may recognize very similar DNA sequences [ 23].
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