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This platform consists of a purpose-made robot that grinds, formats, and dispenses precise amounts of solids into 96-well plates, and a liquid-handling station specifically designed to carry out pretreatments, hydrolysis, and the determination of released reducing sugar equivalents using a colorimetric assay.
The determination of released phosphate was performed with the EnzCheck Phosphatase Assay kit (Invitrogen, CA) according to manufacturer guidelines.
Cellulase activity was estimated by determination of released reducing sugars, after incubation of samples (5 g fresh weight) with carboxymethyl cellulose sodium salt (0.7%) for 24 hours at 50°C, in a Bio-Rad Microplate Reader at 690 nm [45].
Colorimetric determination of released inorganic phosphate (Pi) was then conducted in serial samples.
The starch fraction was hydrolyzed with amylase (E.C.3.2.1.1) and amyloglucosidase (E.C.3.2.1.3), and assayed by enzymatic determination of released glucose [ 60].
GENPLAT utilizes individual purified enzymes, statistical experimental design, robotic pipetting of slurries and enzymes, and automated colorimetric determination of released sugars [ 4, 5].
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A cell culture assay was established for the determination of bioactivity of released IL-18.
We present a framework for the experimental determination of release times and release efficiencies of ISOL-targets devoted to production of neutron-rich nuclei.
Reagent DNS was used for the determination of sugars released during degradation.
Laboratory set for determination of imidazole released from the coordination sphereof Co II): (a) initial preparation, (b) collection of the filtrate.
And this fluorescent nanoplatform was first designed to achieve in situ and real-time determination of H2O2 released from cancer cells to obtain an accurate determination.
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