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Thus, imaging sigma receptors might have potential for predicting prognosis and early diagnosis of the therapeutic effects of the drugs by determining the expression level of the sigma receptor and drug development by determination of receptor occupancy.
We suggest that the automated method will greatly facilitate further progress in the use of AFM for the determination of receptor and multi-protein architecture.
cFC was carried out in C57BL/6j mice and animals were sacrificed in the individual phases and hippocampi were taken for the determination of receptor complex and subunit levels using BN- and SDS-PAGE with subsequent Western blotting.
In addition, the in vivo determination of receptor occupancy allows for the demonstration of target engagement and assessment of titration for potential dose regimens.
For determination of receptor selectivity of the tracer uptake distribution in mice bearing the human melanoma, M21 tumour xenograft as well as blocking with different amounts of H-C*RRETAWAC*OH (200 μg and 300 μg) was studied.
The methodology for the determination of receptor binding affinities for isoform B of the IR (IR-B) and for the receptor of IGF-1 (IGF-1R) is described in the Supporting Information.
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Determination of receptor-status relative frequencies in other African settings, including rural populations, is needed.
Using a Monte Carlo algorithm for the determination of receptor-ligand interactions, they have successfully produced a jerky stop-and-go pattern similar to that observed for rolling leukocytes.
Immunohistochemical determination of receptors was conducted with the use of tests LSAB + kit peroxidase (DAKO, Poland).
Determination of hormone receptor (estrogen receptor and progesterone receptor) and human epidermal growth factor receptor 2 status in the primary tumor is clinically relevant to define breast cancer subtypes, clinical outcome, and the choice of therapy.
Determination of oestrogen receptor, progesterone receptor, ERBB2, Ki67, cytokeratin (CK) 5/6, and epidermal growth factor receptor (EGFR) status by IHC was done according to previously published protocols (Vincent-Salomon et al, 2007).
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