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We then assessed whether test-retest variability and accuracy for determination of proliferation of the population input functions were affected by this reduced sampling population-based methodology.
Besides monitoring tumor metabolism in RCC with [18F]FDG, [18F]FMISO was used to analyze effects on tumor oxygenation status and [18F]FLT for determination of proliferation.
The same ovarian tissue specimens used for determination of proliferation index and DNA content were used for ploidy analysis.
Each determination of proliferation was carried out in at least five replicated wells.
[H]-thymidine was added for another 16 h for the determination of proliferation by scintillation counting.
P53 is a major tumor suppressor gene involved in the determination of proliferation or growth arrest at the cellular level.
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In our study, possible PTX effects on proliferation were examined by immunohistochemical determination of the proliferation marker Ki67.
Cells were harvested at 24 hr intervals for RNA extraction and for determination of cell proliferation by the MTS assay.
Our results show discrepancies when the determination of the proliferation was made either by counting the number of cells or by MTS (figure 1 A and B respectively) for concentrations equal or greater than 6×106 CFU/ml.
For colorimetric determination of cell proliferation, the A549 cells and A549-CEACAM1-4L transfectants were plated in a 96-well cell culture plate at a density of 25,000 cells/well in triplicate.
Determination of cell proliferation, by Ki67-positive cell count in liver sections, revealed delayed progression in the cell cycle in TRα1/TRβ KO and MMI-treated mice (Fig. 2B), while flow cytometry of disaggregated liver cells showed no significant differences in the ploidy distribution among the animal groups (Fig. 2C).
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