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The same complication has to be considered evaluating the UV/VIS spectroscopic determination of products formed during the reaction of PPD with bromine in methanol and in acetate buffer [41].
In each case, the determination of products by fluorescence or HPLC-fluorescence can be even more sensitive and require less expensive instrumentation than the HPLC-MS basedetectionof of Phe and Tyr described here.
After optimization of the hydrolysis (11.5 min, 0.15 M aqueous HCl, 150 °C), the method turns out to be suitable for the determination of products released from quantities of human DNA as low as 500 ng with precision (RSD < 10%) and accuracy (REC 97 104%).
The determination of products (2-methylbutanol, 3-methylbutanol, and isoamyl acetate obtained from Merck, Germany) was carried out by direct injection of samples into a gas chromatograph (Shimadzu GC-14B, Kyoto, Japan) equipped with a split/split less injector and a flame ionization detector as described by Yilmaztekin et al. [ 10].
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These differences in surface speciation and their probable roles in the determination of product selectivity are discussed.
Regulatory science research was first developed for the determination of product safety in the early part of the 20th Century, and continues to support innovation of the processes needed for regulatory policy decisions.
Condensation of tars on the quartz reactor and the pipelines was prevented by an electric heating device, and a temperature control function allowed determination of product characteristics at constant temperatures.
Determination of product yields was not in focus of the study.
1 μl of the amplification product was run on an 0.8% agarose gel for determination of product quality.
A 100-bp DNA ladder was run concurrently with the samples for determination of product molecular weight.
Electron acceptor utilization was tested by determination of product formation from citrate, and in the case of iron (III), by color change of the medium.
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