Sentence examples for determination of primer from inspiring English sources
Additional file 8: Efficiency and R2 values (coefficient of determination) of primer pairs.
Additional file 9: Efficiency and R2 values (coefficient of determination) of primer pairs, when calculated for standard curves (5-fold dilution series from pooled cDNAs) in Chinese Spring (common wheat) and Aegilops peregrina.
Parameters were set by determination of the primer melting temperature and addition of a melt curve to show primer viability.
The polymorphism observed amongst the different ArcB sequences allowed the determination of a primer pair yielding a specific amplification of a 316 bp-long DNA fragment by PCR.
The program Lasergene (DNASTAR, Inc., Madison, WI, USA) was used to align and compare sequences for determination of potential primer binding regions.
Genotyping was performed using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) for the determination of allele-specific primer extension products using Sequenom's MassARRAY system and iPLEX technology (Sequenom Inc).
SNPs were genotyped using MALDI-TOF spectrophotometric mass determination of allele-specific primer extension products with Sequenom MassARRAY platform Sequenom, Inc., San Diego, CA, USA and iPLEX Gold technology (Sequenom, Inc.,).
A ten-fold serial dilution of the cDNA derived from EL-4 cells was prepared in order to make standard curves and determination of PCR efficiency primers for the GM2/GD2 synthase gene (Santa Cruz Biotechnology) and GAPDH housekeeping gene (Evrogen).
Automation of the Determination of Last Exons and Primer Selection procedures was accomplished with software written in Python and Java and employed packages from the site.
Optimization of primers and determination of the input cDNA levels were done to ensure appropriate cycle time response.
Primers, determination of sequence alleles, and designation of sequence types (ST) are described on the MLST web site (http://neisseria.org/nm/typing/mlst/).org/nm/typing/mlst/
