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Generally, the citrulline assay used here is a very sensitive and reliable method for the determination of NOS activity, however it is not suitable to specifically localise NOS activity.
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The Bureau of Ocean Energy Management, part of the Interior Department, said that no competitor had emerged for the right-of-way for the proposed transmission line, known as the Atlantic Wind Connection, allowing the bureau to issue a "determination of no competitive interest".
The plasma was stored at -70°C for subsequent determination of NO.
For a determination of NO concentration, the amount of nitrite in the cell culture supernatant measured by using Griess reagents.
The heart and aorta were collected, weighed and used for both histological analysis and determination of NO tissue content.
Uncertainty as to consequence, so seek a determination of no difference between the transgenic and wild-type enzyme, in order to be conservatively protective of ecological-value.
Determination of NO in the cell culture supernatants was performed in duplicates in 96 well plates using the method of Green et al., [ 41].
The basal activation decreased after treatment of the cells with 100 μg/ml gentamicin for 2 h before determination of NO production, but remained high when H2O2 and TNF-α were measured.
The cells were washed after overnight incubation, and the culture continued for 24 hours in MEM, 0.5% fetal bovine serum, with or without L-NMA, agonists added, and conditioned media for determination of NO production collected 24 hours later.
All collected samples will be mixed 10 s before the determination of NO concentration by chemiluminescence (Sievers 280 NOA; Sievers Instruments, USA) and analyzed up to 24 h after sample collection.
The determination of CSF-NO metabolites could be useful to clarify whether increased NO production is predominantly associated with poor perfusion of the brain in preeclampsia.
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