Sentence examples for determination of mutant from inspiring English sources
For determination of mutant receptor surface expression, transiently transfected cells were analyzed 48 hr after transfection.
TILLING consists of a series of steps, beginning with chemical mutagenesis of reference individuals and culminating in the determination of mutant base pairs by DNA sequencing [ 14].
Determination of the mutant prevention concentration (MPC), the antimicrobial concentration that prevents selection of resistant mutants, is important for reduction of the incidence of fluoroquinolone resistance [ 4].
A structure determination of the mutant in complex with an ATP-analogue confirmed the predicted change of the purine base binding plane orientation.
Variation among lineages in terms of nucleotide diversity during chicken passage makes determination of representative mutant spectrum size and clear evaluation of selective pressures difficult.
ARMS strategy (amplification refractory mutation system) also implies the use of fluorescent dye for the quantitative determination of the mutant allele by real-time PCR.
All the other mutations lead to a reduced number of spots, which could be expected from the copy-number determination of these mutants.
Sequence determination of the mutants led to the development of techniques to exploit the polymerase chain reaction, which, together with Southern analysis, have been reported to detect as many as 95% of poor metabolizers.
Thirty samples with a WT/WT genotype by the reverse line blot assays were analysed subsequently by DGGE and sequencing that resulted in only two further determinations of the mutant genotype.
We demonstrate the atomic basis of insecticide resistance through structure determination of the G119S mutant acetylcholinesterase of An. gambiae in the ligand-free state and bound to a potent difluoromethyl ketone inhibitor.
The utility of this approach is highlighted by the automated determination of a lysozyme mutant from a molecular replacement solution that is in congruence with a model prepared independently by crystallographers.
