The determination of microorganism pathogenicity determinants according to the classification proposed by Casadevall and Pirofski (six classes) poses some interpretation problems in class I, since it seems that the key role in these infections is played by a host's condition.
The use of surfactants result in forming aggregates in the system with bacteria:hydrocarbons:surfactant which disrupts the determination of microorganism hydrophobicity using sedimentation method.
Therefore, determination of hospital microorganisms, and selection of relevant disinfectant active on these microorganisms is useful to prevent, and control hospital infections [ 29].
With the development of Phenotype microarrays (PMs), high-throughput determination of a microorganism's global metabolic phenotype or phenome is now possible [ 33– 33].
A modification of the procedure was proposed by Brun-Buisson et al. in 1987 [ 8], which consisted of diluting the bacteria in sterile water followed by vortexing for determination of the presence of microorganisms on the external and internal surface of the catheter.
According to ISO 11737 1 2006 for the sterilization of medical devices, both of the content including determination of a population of microorganisms on product and tests of sterility performed in the validation of a sterilization process was not determined as our practical culture method which was designed before the documentation of ISO 11737 1 and ISO 15883 4.
A novel method for determination of trace amounts of microorganism populations in solution was developed by using a multiwalled carbon nanotube (MWNT) modified glassy carbon electrode and square wave voltammetry (SWV).
Determination of minimum inhibitory concentrations (MIC) of microorganisms was performed using the method of microdilutions on 96-well plates according to obligatory methodology [ 37].
Additional quality control of I'm-Yunity™ (PSP) involved determination of heavy metal contents, microorganism contamination, authentication of elution profiles on high pressure liquid chromatograph, and supplementary bioactivity-guided assays, performed by laboratories at the manufacturing facilities and also independently as coded samples by commercial testing centers.
Determination and quantification of basic physiological groups of microorganisms in rhizosphere soil of chickpea plants was performed using standard microbiological methods [14].
