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The determination of linkage by individual adjudicators was highly consistent, with identical independent assessments in 96% of cases, followed by full concurrence after discussion.
The LOD score and recombination threshold for the determination of linkage groups were, respectively set at 3.5 and 0.4.
A second classical strategy is cloning via mapping using determination of linkage with a large number of genetic markers on all chromosomes in meiotic tetrads.
These common markers enabled the determination of linkage group orientation and assignment of linkage group numbers for the Pop-DG map.
Outputs from QMSim included information about the paternal and maternal alleles of each locus, which allowed the determination of linkage phase and the calculation of haplotype frequencies.
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Determination of linkages among contigs and gap closing was carried out as previously described [94] except that repetitive reads were put back into the contigs by separated bin assemblies.
This work is in progress and when done would lead to the determination of the linkage between markers and their position on mulberry linkage groups.
Genotyping the grandparents allows determination of the linkage phase of the parents using identity by descent and yields improved results where there is limited marker coverage over inferring phase based on independence LOD scores of marker pairs.
This will facilitate a faster and more precise determination of the linkages among contigs and greatly improve the efficiency of gap closing.
Displaying straightforward graph-based relationships of contigs in Cytoscape rather than tables also facilitates a faster and more precise determination of the linkages among contigs.
The determination of genome-wide linkage disequilibrium (LD) patterns through the HapMap project has enabled the selection of markers for efficient genome-wide association (GWA) studies in human samples[ 8].
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