The 99mTc labeling of BN-PEG and determination of labeling yields were performed [17] (99mTcO4 − was purchased from the China Institute of Atomic Energy, Beijing, China; 5 mCi).
The 99mTc labeling of oMWCNTs/NDs and determination of labeling yields were preformed (99mTcO4− was purchased from the China Institute of Atomic Energy, Beijing, China; 5 mCi) according to the method of Li et al. [5].
Experimental design of 13C-tracer studies for metabolic flux analysis with mass spectrometric determination of labeling patterns was performed for the central metabolism of Corynebacterium glutamicum comprising various flux scenarios.
In the late exponential phase, biomass samples from the cultivations were harvested, hydrolyzed, derivatized and analyzed by gas GC-MS for determination of labelling patterns of intracellular metabolites.
Regarding the determination of labeling yields, those methods which allow the simultaneous separation and detection of nonlabeled and labeled glycans have to be considered.
For determination of labeling patterns of lactate and amino acids, 50 μl of supernatants were lyophilized, resolved in 50 μl N,N-dimethylformamide (0.1% pyridine) and incubated at 80°C for 30 min.
Detection was maintained parallel by the use of RP-ESI-MS (applying a Zorbax 300 SB C18 column) for determination of labelling completeness and yield, and RP-ICP-MS utilizing the same column for gathering of relative quantitative information (Fig. 3).
At 14 or 18 weeks of age, distribution volume determinations of labeled erythrocytes and 40 kDa high molecular weight dextrans were performed to assess systemic vascular red blood cell- (Vrbc), plasma- (Vplasma), surface layer- (Vesl) and total vascular volume (Vtotal) as previously described (20, 31, 36).
For the label propagation problem, we usually have the following hypothesis: a data sample and its nearest neighbors usually belong to the same class, and the nearest neighbors would have a big influence in the determination of the labels of new data samples.
How should the new capability, determination of isotopic labeling states of heteronuclear moieties, be leveraged?
