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Practical diagnostic applications of such assays demonstrated in this study include: (1) rapid genotype determination of isolates; and (2) identification of mixed-genotype isolates originating from pooled samples in areas where genotype distribution is known to overlap.
More methods provide possibilities for more laboratories to perform serotype determination of isolates, which is of high value when estimating and evaluating vaccine efficacies and vaccine failures.
DNA was isolated by suspending 1 loop of bacteria in 200 μl Tris-EDTA (TE) buffer, pH 8.0, heating at 95°C for 10 minutes and centrifugation at 16,000 × g for 5 min. Capsule gene PCR [ 36] was applied for serogroup determination of isolates non-serogroupable by slide agglutination.
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In this research a practical method has been utilized based on determination of isolated reliabilities.
Therefore, rapid diagnostic determination of isolate genotype and phenotype is important.
But FUNAI officials and others have argued that a no-contact policy is vital to the health and self-determination of isolated people.
Detection and species determination of Candida isolates are performed in the notifying laboratories according to standard protocols in use in each laboratory.
A total of 53 post repair obstetric fistula patients were included for the determination of bacterial isolate and 28 (52.8%) of them had significant bacteriuria.
Rather than making the determination of relationships among isolates more confusing, however, alternate alleles reveal the potential mutations that connect isolates.
Culturing of Clostridium difficile was done by BK and virulence gene determination of E. coli isolates by ØA.
Determination of susceptibility revealed isolates resistant to fluconazole (4.8%), 5-flucytosine (1.6%) and amphotericin B (3.2%); the stratification of sensitivity results for each species showed significant differences in susceptibility to azoles.
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