Gene knockout is the most powerful tool for determination of gene function or permanent modification of the phenotypic characteristics of an animal.
Cells were collected for the determination of gene expression levels and then used in bioconversion using resting cells for another period of 2 days.
This will strongly complement an iterative approach to the evaluation of capsid variants in the clinic and, should wherever possible, include the determination of gene transfer efficiencies.
Nuclear medicine procedures may be applied for the determination of gene function and regulation using established and new tracers, or using in vivo reporter genes, such as genes encoding enzymes, receptors, antigens, or transporters.
cDNA was used for determination of gene expression level using TaqMan Low Density Arrays (Applied Biosystems).
The standard curves for the determination of gene numbers were established in the same manner described previously [47], [48].
The biological theme determination of gene lists in DAVID are based on the Expression Analysis Systematic Explorer (EASE), a variant of one-tailed Fisher exact probability [25].
Marker-based prediction is usually more accurate than transcript-based prediction, likely reflecting the genetic determination of gene expression in this cross.
Gaps larger than 500 bp were examined on the fully sequenced genome of P-SSM2 in GenBank for determination of gene content.
For determination of gene expression Assay-on-demand (Applied Biosystems) was used (Hs00159163_m1 for MMP7, Hs00181225_m1 for FasL, Hs_00531110_m1 for Fas), which consist of a 6-FAM dye-labeled TaqMan MGB probe and the corresponding unlabeled primers.
The released chondrocytes were subject to determination of gene expression.
