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Hence, to validate expression and assist with the determination of functions, the expression for the 63 miRNAs in 8 different organs (gill, brain, eye, muscle, liver, intestine, heart and kidney) and in spleen following 24 h post-challenge with LPS and control sample were examined by qRT-PCR (Figure 5).
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New procedures are necessary to facilitate this research and improve determination of function for all the DUF proteins.
To test this approach, we developed an animal model that enabled simultaneous measurement of cardiac function with a transonic flow meter and PET determination of function using tracer kinetic models which utilizes the sinogram binning.
This indicates that determination of function of NRT2 genes in grass species will not be possible in cereals based simply on sequence homology to functionally characterised Arabidopsis NRT2 genes and that proper functional analysis will be required.
24 Discovering sequence motifs or repeated patterns is of greater biological importance because its identification can lead to determination of function and to elucidation of evolutionary relationships among sequences.
This suggests a tantalizing possibility that links are also expressed very early in development, perhaps at very specific stages of development, which explains why they so far have eluded detection and the determination of function during early development.
The clustering of all members into well-defined subfamilies and detailed analysis of features characteristic for these subfamilies will help to select representatives for the experimental determination of function and structure and will facilitate the classification of novel members identified in the future.
The algorithms for generating SALCs and symmetrising molecular orbitals include the generation of vector spaces for irreducible representations, using real spherical harmonics basis functions and determination of partner functions.
There is an increasing interest in the determination of their function, role in plant-pathogen interactions and molecular structure [ 4].
Many of the problems derived from the determination of harmonic functions on a class of Markov chains.
The detailed information on the development of constitutive relations and determination of prediction functions of material properties for the fully coupled THMC model are provided in (Cui and Fall 2015a).
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