Sentence examples for determination of cell number from inspiring English sources

Determination of cell number per colony size under tested conditions was performed to control for putative differences in adhesive capacity, which might impact on measurement of clonogenic cell survival.

Following determination of cell number, neurons were plated on poly-D-lysine-coated coverslips within 24-well plates for immunocytochemical studies (seeded at a density of 2.5×104 cells/coverslip), or seeded on poly-D-lysine coated 6-welll plates for immunoblotting at a seeding density of 2.5×105 cells/well.

Two tubes without labeled dexamethasone were incubated under the same conditions for determination of cell number and viability at the end of the procedure.

For the determination of cell number (DNA amount), cell layers were washed with PBS and frozen with 1 mM Tris HCl buffer (pH 8.0) containing 0.1 mM EDTA.

Following determination of cell number the cells were, soulbilised with 1% tritonX/PBS for 5 mins, and blocked in 100 ul PBS containing 10% BSA for 30 mins.

Cell were trypsinized, and after three washes with phosphate-buffered saline and the determination of cell number, the final cell pellet was suspended in Hanks solution to a final density of 2 × 10 4 × 10 cells/ml.

For determination of cell numbers at time of infection, six wells of one six-well plate were counted and averaged.

An inherent problem of infection experiments in static cultivation vessels, like six-well plates, is the determination of cell numbers.

The 2D model is advantageous for quantitative experiments, in that it is amenable to measurement and determination of cell numbers.

*pc = postcolostral ** with few exceptions due to laboratory shifts Determination of cell numbers was performed using the semi-automated haematology analyser Sysmex F-820.

An alternative cell viability test system, the CyQUANT assay is a sensitive technique used for the determination of cell numbers in culture, and unlike MTT or MTS, this assay does not depend on cellular metabolic activity.