Even though the assumptions on which the value of b is based (eq 55) can be questioned, the determination of a structure-independent atomic volume and a characteristic length for each element is impressive, and the calculated atomic valences and values of b seem highly plausible, even if their meaning in a metallic context is not clear.
It is possible that determination of a 3D structure of IsdBN1LN2 could reveal important structural changes within IsdB linker domain that could explain its effect on heme transfer from metHb.
The first complete determination of a protein structure with Wüthrich's method was achieved in 1985, and about 20 percent of protein structures known by 2002 had been determined with NMR.
Three technological breakthroughs also appear on our list: functional brain/machine interfaces, TALENS as a tool for genetic engineering, and the x-ray laser determination of a protein structure.
We have identified crystallographic conditions that led to the determination of a crystal structure of the sarinnonaged-conjugated mouse AChE in complex with [(E -[1-[ 4-carbamoylpyridin-1-ium-1-yl)mE -[1-[ 4-carbamoylpyridin-1-ium-1-yl]-oxoazanium dichloridE -[1-[ 4-carbamoylpyridin-1-ium-1-yl
The determination of a crystal structure of a substrate complex for de novo initiation provided a firm foundation for structural modelling of relationships between GE and initiating nucleotides in a transcription initiation complex.
The interconversion of these two conformational equilibria at an intermediate rate on the NMR time scale, evidenced by the broadening and loss of key NOEs, precluded the determination of a refined structure of the 1, N-ϵdG adduct at pH 5.2.
However, there is no widely used NMR approach similar to the crystallographic methodology of crystal soaking and model building through a difference Fourier map, bootstrapping the determination of a protein−ligand structure through the assumption of a small perturbation to a known one.
The precise role of His48 as a possible proton donor awaits the outcome of other experiments, including the determination of an HbpA structure in the presence of the substrate or product.
The identity and stability of the product(s) are determined by various analytical chemical approaches that allow determination of a substance's structure on the basis of its spectral properties and reactivity.
