Following determination of DNA concentrations using PicoGreen [ 22], pools containing equimolar amounts of DNA from each contributing sample were created and used for PCR.
For the quantitative determination of DNA synthesis rate, mitogenic activity was determined by measuring BrdU incorporation using BrdU Cell Proliferation ELISA (Roche Diagnostics, Indianapolis, IN).
Thus simple and sensitive determination of DNA MTase activity is urgently required.
DNA from the aliquot before immunoprecipitation was serially diluted and used to generate a standard curve for quantitative determination of DNA binding data calculated as % input.
Recent technological developments have enabled determination of DNA trajectories in a few DNA-loop-containing regulatory complexes.
The decrease in the SWV peak current of GCV in the presence of DNA was used for the determination of DNA.
Specifically, N7-methyl modification of guanine (7-mG) is well established to be used as a biomarker for the detection and determination of DNA methylation.
This paper should have opened a new era for the sensible determination of DNA synthesis kinetics, however, by this time people were tired of and/or confused by this type of assay, and radiation biology shifted to new procedures.
We report here the determination of DNA sequences for Meganyctiphanes norvegica (Crustacea, Euphausiacea) preserved and stored in buffered formalin for up to 25 years.
A novel and sensitive electrochemical biosensor for selective determination of DNA was developed based on Fe@Au nanoparticles (Fe@AuNPs) involving 2-aminoethanethiol (AET) functionalized graphene oxide (GO) (Fe@AuNPs-AETGO).
The suggested biosensors were applied for determination of DNA sequences and showed excellent selectivity for discriminating the complementary sequences from various targets such as non-complementary, one base-mismatch and three base mismatch sequences.
