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Glycerol level was determined using Free Glycerol determination kit according to the manufacturer's instruction (Sigma, USA).
Cellular ATP levels were determined using the ATP determination kit (Molecular probes, Invitrogen) according to the manufacturer's instructions.
The ATP content was determined using the ATP Determination Kit (Molecular Probes, Eugene, OR, USA).
The eluted fractions were pooled, and the protein concentration was determined using a protein determination kit (micro BCA kit; Pierce) according to the manufacturer's protocol.
Adenosine triphosphate (ATP) levels were determined with an ATP determination kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's protocol.
Serum level of triglycerides was determined by the Serum Triglyceride Determination Kit (Sigma).
Triglycerides were determined by the Serum Triglyceride Determination Kit (Sigma-Aldrich).
Protein concentration was determined by BC assay protein determination kit (Interchim).
Adenosine tri-phosphate content was determined by using an ATP Determination Kit (Molecular Probes, Grand Island, NY, USA).
The levels of fasting triglycerides were determined by the serum triglyceride determination kit (Sigma-Aldrich, St . Louis MO).
ATP was determined using a time-stable ATP determination kit (Biaffin) based on the luciferase assay.
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