The coefficient of determination is 0.817 and R2 is 0.667, which indicates that the regression is statistically significant.
The limit of determination was 0.034 μmol l−1 which shows high sensitivity of the developed method.
The linearity was examined in the range LOQ-60 μg/mL and the coefficient of determination was 0.998.
The practical working range of the determination was 3 200 ng/mL with detection limit of 1.63 ng/mL.
The values of limits of detection and determination were 0.1 3.3 ng mL−1 for heptane and 0.3 10.7 ng mL−1 for tert-butyl acetate.
The linear range for germanium determination was 0.1 10 ng mL−1 for 1 mL sampling volume with a detection limit (3 s) of 0.01 ng mL−1.
The regression equations of RBC, WBC and Hb toward the two factors of interest were established, with the coefficients of determination being 0.870, 0.836 and 0.881, respectively (P < 0.01).
The regression equations of pituitary GH, hepatic GHR1 and IGF-I mRNA towards the two factors of interest were established, with the coefficients of determination being 0.958, 0.938 and 0.978, respectively (P < 0.01).
The operating conditions employed for ICP-OES determination were 1,300 W RF power, 15 L min−1 plasma flow, 2.0 L min−1 auxiliary flow, 0.8 L min−1 nebulizer flow, and 1.5 mL min−1 sample uptake rate.
The accuracy and precision for MT determination were 102.3%–107.2% and 6.5%, respectively.
