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Response rates were not affected by method of HER2 status determination (FISH v IHC) or by prior adjuvant chemotherapy.
After sex determination, fish were terminally anesthetized with MS-222 and discarded.
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In this context, the OECD 305 guideline [1] recommends the use of chloroform/methanol for determination of fish lipid content.
Further information and tools for high-quality sampling are provided in two appendices: Appendix I focuses on age determination of fish using gill covers (opercula) or further ossifications.
S. putrefaciens count is probably not only a sufficient parameter for shelf life prediction, but is also useful in quality determination of fish.
Recently, FT-IR spectroscopy was applied in aquaculture to investigate the chemical components in white sturgeon female plasma, leading to a rapid and less-invasive determination of fish maturity Lu et al. (2010, 2013).
Due to the specific extraction procedures required for the analysis of many test substances, the use of techniques based on NIR or NMR spectroscopy (e.g., CEM Smart trac™, CEM Corporation, Matthews, NC, USA) has high potential for the lipid determination in fish sampled from bioaccumulation studies.
Nevertheless, the precise mechanisms involved in primary sex determination of fish have not been studied extensively.
This then may contribute to a broader understanding of the complex and diverse pathways of sex determination in fish.
However, despite these limitations, this situation did not prevent that TSD was until now considered a widespread mechanism of sex determination in fish.
In addition the finding of the QTL for weight and the QTL for sex at the same genomic position is in line with newer synthesis about sex determination in fish.
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