The programs used for the crystal-structure determination are – Data collection : APEX2 [26], Cell refinement : APEX2 and SAINT [26]; data reduction : SAINT and XPREP [26]; structure solving : SIR92 [27] ; structure refinement : SHELXL97 [28] ; molecular graphics: ORTEP [29] and Mercury [30].
The authors' responsibilities were as follows: LT, AMPG and IW implemented the recruitment and contributed to the analytical determinations and data collection; LT and MPV contributed to the manuscript preparation; LT, AMPG, RBR, IW and MPV contributed to the questionnaire design; and MPV was the principal investigator of the study.
The second stage is the determination of the data collection strategy and a selection of multiple channels in order to avoid a bias in coverage (Section 2.1).
Initial indexing and determination of an optimal data collection strategy was done using Mosflm (Leslie, 2006).
Details of the synchrotron data collection, structure determination, and refinement statistics are presented in Table 2. Superpostition of the different crystal structures illustrates the location of flexible regions (Figure 1A, 1B).
Crystallization, data collection, structure determination and refinement.
JAG and IPD carried out the diffraction data collection, structure determination.
Details of the data collection, structure determination, map quality, and refinement are presented in Table 1 and Figure S2.
Stock solutions were then diluted in buffer to 10 μM for data collection and determination of the thermodynamic parameters for siRNA unfolding.
Our study highlights the importance of standardisation of the methods used for storage and staining of tumour tissue as well as the patient population, data collection and determination of clinical end points.
