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All calculations [averages, standard deviations, slopes, coefficients of determination, copy numbers, and % coefficient of variations (CVs)] were calculated using Microsoft Excel (Redmond, WA).
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A The workflow of AQUA strategy for determination of protein copy number per ZG.
Determination of SMN1 copy number in a general carrier screening population permits identification of the majority of SMA carriers before the birth of an affected child.
We report an optimized method for the determination of copy number variation (CNV) in GST genes.
Unfortunately, our marker technology did not enable the quantitative determination of copy numbers to confirm homozygous disomy.
It is conventionally used for absolute quantitation ("molecule counting") of genomic DNA and cDNA, rare mutation detection [18], and determination of copy number variation [20], [21] (CNV).
The determination of copy number of Avr1a and Avr3a sequence intervals by depth-sampling was reliable and concordant with results from other methods, such as Southern blot hybridization and real-time quantitative PCR.
For determination of copy numbers of Tg40, Tg42 primer pairs p60 (5'-CGC TAC CCT AAC CAA GTG T) and p61 (5'-GAT CTT CTC CCG TCG TAA T) were used.
Determination of copy number variation by quantitative real time PCR was performed using the Applied Biosystems 7900HT Sequence Detection System and analyzed with the SDS 2.2 software following the guidelines of the manufacturer (Applied Biosystems).
It has recently been proposed that SNP arrays may be better suited for the determination of copy number states in tumor samples because the analysis of data derived from such arrays can make use of allelic imbalance information in addition to hybridization intensity [12].
This correction permitted determination of copy number differences between the samples under investigation.
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