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The linear range of urea determination by using the biosensor was 0.05 15 mM, and a lower limit of urea detection was 20 μM.
After 48 hours incubation, the cultures supernatants were collected for NO determination, by using the standard Griess reaction, in which the micromolar concentrations of NO were deduced from a standard curve using a known concentration of NaNO2 diluted in RPMI medium.
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A sandwich-type immunoassay format was employed for determination of AFP by using the labeled HRP toward the reduction of H2O2 with the aid of thionine in pH 6.5 acetic acid-buffered solution.
Protein determination was made by using the BCA™ Protein Assay Kit (Pierce).
Phase determination was performed by using the SOLVE program [17] on two wavelengths corresponding to the peak (0.979774 Å) and the inflexion point (0.979958 Å) in the 43.437 to 2.341 Å resolution range.
Graphic summary of the extent and significance of LD determinations was displayed by using the program HaploView version 4.0 [ 65].
MIC determinations were performed by using the E-test (AB Biodisk, Solna, Sweden) on Iso-Sensitest agar.
In the present study, we performed multivariate analysis for determination of MDA-LDL by using the factors of LDL-C level, age, BMI, HbA1c, Cr, BNP and Brinkman index; however, there may be other factors associated with MDA-LDL that act in a direct or indirect manner.
Determination of principal dimensions begins by using the ratio of principal dimensions from past experience.
Then we discuss a preliminary temperature determination for the jet plasma by using the filter ratio method at 171 and 195 Å and applying a technique for subtracting the EUV background radiation.
This result indicates that the determination of human muscle V0 by using the slack test is as reproducible as the muscle strength measurement that has been commonly used in the area of human muscle physiology.
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