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Targets for protein production by the Protein Core of the Enzyme Function Initiative (EFI; enzymefunction.org), functional characterization by the University of Illinois, and structure determination by the Structure Core of the EFI were chosen from both types of genome neighborhoods.
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The analysis proposed is based solely on equilibrium data and is illustrated by determination of the structure of the apoflavodoxin thermal unfolding intermediate.
A direct test of this hypothesis can be expected by determination of the structure of a complex of F1 TnC with TnI in the absence and presence of calcium.
The power of this approach is illustrated, step-by-step, with the determination of the structure adopted by a potential G-quadruplex sequence found in the 5′-untranslated region of the cAMP responsive element modulator (CREM) mRNA.
Recently, however, the crystal structures of isolated alpha and beta chains were determined, shortly followed by the determination of the structure of an alpha beta heterodimer.
We have first addressed the determination of the structure by calculation of the cylindrically averaged intensity transforms of single chains averaged overall possible sequences.
This was confirmed by the determination of the structure of CALMANTH 1-264 CALMANTH 1-264ution (finat R/Rfree 0.1.7Å0.191; see Table S1).
This behavior of CHA was proven by the isolation and determination of the structure of cyclohexylammonium nitrate crystals by single-crystal XRD[33].
A more detailed picture of the lid architecture has been provided by the recent determination of the structure of the S. cerevisiae 19S RP [ 20] and of the Schizosaccharomyces pombe 26S proteasome [ 21] by electron microscopy methods.
The RdRps of dsRNA viruses and negative-strand RNA viruses are likely to be highly diverged derivatives of the same polymerase domain, an old conjecture [ 24- 26] that has been vindicated by the recent determination of the structure of a dsRNA bacteriophage RdRp [ 27, 28].
Such determination may be prevented by the structure of the model equations as such (lack of structural identifiability) or, more generally, because the model structure and/or the scarcity or poor quality of the data make it impossible to obtain reliable parameter estimates (lack of practical identifiability) [ 44].
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