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Protein concentration was determined using the Bio-Rad protein determination assay reagent.
Urine creatinine was determined using a quantitative colorimetric creatinine determination assay (QuantiChrom™ creatinine assay kit-DICT-500) (BioAssay Systems, Hayward, CA, USA) as described below for mice.
Serum and urine creatinine were determined using a quantitative colorimetric creatinine determination assay (QuantiChrom™ creatinine assay kit-DICT-500) (BioAssay Systems).
Protein content of cell lysates was determined using the bicinchoninic acid (BCA) protein determination assay (Pierce Biotechnology, Rockford, IL, USA).
The lead peptide was then tested against a panel of fluconazole-resistant C. albicans, subjected to a fungicidal/static determination assay, a human dermal fibroblast viability assay and a homozygous profiling assay to gain insights into its mechanism of action and potential for further development as a topical antifungal agent.
Lysate protein concentration was adjusted to 2-5 mg/ml following a colorimetric protein determination assay (DC Protein Assay, Bio-Rad).
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All IC50 determination assays were done in duplicate and the IC50 values were calculated using Prism (GraphPad Software , Inc.
The purpose of the present study was to improve HIT recognition by the combination of functional and antibody determination assays.
Fluorescent dyes, such as ethidium bromide, which is routinely used in cell viability determination assays, are transported by the three human OCT isoforms [ 63].
For the kinetic determinations, assay linearity was confirmed under all conditions.
Cortisol values are expressed as mean±SEM of duplicate independent determinations, assayed in duplicate.
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