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And then, all of a sudden, she was no longer the small, apologetic woman, vague and cheerfully flustered; she had gleaming eyes, a stubborn mouth and an air of utter determination and concentration.
Farnes recalled Van der Bijl as a player of "great determination and concentration", "an excellent judge of the ball to leave alone, he was very hard to shift indeed".
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It was an expression of determination and fierce concentration; when Lyndon Johnson wore that expression, a problem was being thought through with an intensity that was almost palpable, a problem was being thought through — and a decision made.
One RNA transcript will be chosen as a standard for concentration determination and the relative concentrations of other RNA transcripts will be determined based on this standard.
For the determination of SELENBP1 mRNA levels RNA purification, determination of purity and concentration and cDNA synthesis were carried out as previously described [ 43].
The determination of PCR efficiency, threshold cycle (Ct) determination, and RNA starting concentration were calculated using raw fluorescence data using LinRegPCR analysis software [75], [76].
Accurate determination of the size and concentration of nanoparticles is essential for the biomedical application of nanoparticles.
The induction, purified and concentration determination of recombinant protein were performed as described previously [ 46].
Protein extraction and concentration determination were the same as described in ELISA.
Aliquots of 0.5 ml were then removed for butanol extraction and concentration determination.
Labeling reaction components and incubation conditions as well as cDNA probe purification and concentration determination have been described previously [ 26].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com