The conventional watershed simulation process includes sink filling, flow direction determination, flow accumulation and stream definition (Zhang and Huang 2009).
g-h Determination of ROS accumulation in leaves.
Following reperfusion, the lungs were removed for microscopic determination of neutrophil accumulation.
This allows the precise determination of the accumulation time in the bladder and, hence, the determination of the urinary flow rate, creatinine excretion rate and metabolite excretion rates.
For strains used in this study, mutant-strain construction, growth conditions, and determination of Ca2+ accumulation, see the Supplemental Experimental Procedures.
These cells were induced to differentiate, and the ability to undergo differentiation to mature adipocytes was evaluated by determination of lipid accumulation using Oil Red O staining.
The potato leaves were inoculated with 20 μL zoospore suspension (5 × 10/mL) and incubated at 20°C with 85% humidity for resistance evaluation and determination of H2O2 accumulation [ 78].
These cells were induced to differentiate, and the ability to undergo differentiation to mature adipocytes was evaluated by the determination of lipid accumulation using Oil Red O staining and determination of relative mRNA levels of adipocyte-specific genes.
In the current study, three main issues were therefore addressed: i) Determination of the accumulation and translocation of TCDD in the tissues of Arabidopsis during a whole growth cycle.
These cells were stimulated to differentiate, and the ability to undergo differentiation to mature adipocytes was evaluated by determination of lipid accumulation using Oil Red O staining, direct measurement of intracellular triglyceride contents and determination of relative mRNA levels of adipocyte-specific genes including Fabp4, Cidec, Klf15, Adipoq, and Retn.
