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For sex determination, a minimum of three 1 mM sections of mesenteries from each specimen were prepared as squashes on microscope slides analysed.
In each determination, a minimum of 200 cells was evaluated.
For CRM determination, a minimum of two sections were taken of this area with minimal circumferential distance and stained with hematoxylin and eosin (H&E) for microscopic examination (Fig. 2).
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Mice used for glycogen determination were non-exercised for a minimum of one week and euthanized within 4 hours after the beginning of the light cycle.
The sample size was in accordance with the rigorous CLSI guidelines for determining laboratory RIs, which recommend a minimum of 120 subjects for determination of a 95th percentile clinical reference range [ 9].
For determination of percent TUNEL positive cells, a minimum of 9 different fields per group were counted at 20× magnification for TUNEL positive cells and total cell number.
All the results of spectrophotometric determinations of flavones and polyphenols were reproduced after establishing the confidence interval calculated for an average of a minimum of five determinations.
Inter-run accuracy should be measured using a minimum of three determinations per concentration.
Concentration-response curves were calculated using the Prism software package 5.00 for Windows, GraphPad Software, San Diego, CA, USA, http://www.graphpad.com/ (GraphPad, San Diego, USA) and data were reported as mean and SD values obtained from a minimum of three determinations.
Patients were to monitor their fingerstick glucose throughout the study by performing a minimum of two premeal determinations per day, with additional monitoring during titration periods or at an investigator-determined frequency.
Blood samples were measured a minimum of five times for determination of %HbCO using an OSM three hemoximeter (Radiometer, Copenhagen).
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