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The nucleotide sequences of conjugative plasmids with Dutch type plasmid backbones either not containing the tetM determinant (pEP5233) or containing Dutch (pEP5289) or American (pEP5050) type tetM determinants were determined.
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The genetic location of their resistance determinants was determined by curing and transfer experiments.
The presence of virulence and AMR determinants was determined by BLAST against reference loci as described previously (Reuter et al., 2014).
The sequence of the Dutch type backbone, and the sequences of the genetic load regions in which the Dutch and American type tetM determinants were inserted were determined.
The independent determinants of a variable were determined by multiple linear regression analysis.
The presence and distribution of 11 antibiotic genetic determinants from individual wastewater bacterial isolates were determined by the conventional polymerase chain reaction (PCR).
Following immunization of mice at the lower dose (106 PFU), MVAΔudg elicited 14-fold and 9-fold lower frequencies of CD8+ T cells against the A3L270 277 (Late) and A19L47 55 (Unknown) determinants, respectively, than did MVA, which were determined to be statistically significant differences (Mann-Whitney test).
The determinants of hyperglycaemic emergecncies' associated mortality were determined using logistic regression.
Antibiotic susceptibility profiles and associated genetic resistance determinants, and the presence of putative virulence factors were determined.
Genetic relationships were determined by PFGE, and isolates were screened for selected resistance determinants.
Optimal conditions were determined using an analytic method, stationary point of model was determined, and the determinant values of Hessian matrix showed that the response (yield of AMX elimination) agrees a maximum solution corresponding to optimal conditions, Tc = 488 °C, T = 33.6 °C, [AMX] = 58.5 mg·L-1 and [AC] 1.178 g·L-1, at which the predictive value of the response is ye = 100±3%3%.
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